<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2022 Workiva-->
<!--r:34b53e79-3e78-4863-984e-66abe1fb862c,g:565677df-ba47-4369-80ed-4b4b07e63ca8-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedBalanceSheets" xlink:type="simple" xlink:href="aqst-20220630.xsd#CondensedConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_df4b6058-0c49-4a15-9f71-c182ebc41b89" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_df4b6058-0c49-4a15-9f71-c182ebc41b89" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_a14cf9f0-9c7e-4e0b-ab30-e84929bf0ecc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_a14cf9f0-9c7e-4e0b-ab30-e84929bf0ecc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_f3905dba-71d4-414d-89dd-53dc4ef0e30c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:to="loc_us-gaap_AssetsCurrent_f3905dba-71d4-414d-89dd-53dc4ef0e30c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_1524b07c-64c8-416c-95c6-38792c29619b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_1524b07c-64c8-416c-95c6-38792c29619b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_c9f4a2ef-8605-4d89-b2df-33a808a38bd6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_ff34a5c4-f2c7-4c8b-8bbd-01dd27043708" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_c9f4a2ef-8605-4d89-b2df-33a808a38bd6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_97562844-cde0-4c40-b5ea-74fda767e8aa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_5bc23f7a-ec66-4af8-b99b-4788e38ff424" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_97562844-cde0-4c40-b5ea-74fda767e8aa" xlink:to="loc_us-gaap_StockholdersEquity_5bc23f7a-ec66-4af8-b99b-4788e38ff424" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_aa951dca-f63a-44bc-a4be-f32e23cf33d4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_97562844-cde0-4c40-b5ea-74fda767e8aa" xlink:to="loc_us-gaap_CommitmentsAndContingencies_aa951dca-f63a-44bc-a4be-f32e23cf33d4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_eb93bd1c-4048-4c92-a370-7016f2a83c11" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_97562844-cde0-4c40-b5ea-74fda767e8aa" xlink:to="loc_us-gaap_Liabilities_eb93bd1c-4048-4c92-a370-7016f2a83c11" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_7f853972-a48d-40f6-883e-7fff8fee8e2f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_832cfc39-cc7b-49c3-b668-65bb5c055cf9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_7f853972-a48d-40f6-883e-7fff8fee8e2f" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_832cfc39-cc7b-49c3-b668-65bb5c055cf9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_6b464798-e399-499c-a7d1-4884de792259" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashAndCashEquivalentsAtCarryingValue"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_7f853972-a48d-40f6-883e-7fff8fee8e2f" xlink:to="loc_us-gaap_CashAndCashEquivalentsAtCarryingValue_6b464798-e399-499c-a7d1-4884de792259" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_aaad839a-b8bc-4a60-98f6-36ea5ebbb3df" xlink:href="aqst-20220630.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_7f853972-a48d-40f6-883e-7fff8fee8e2f" xlink:to="loc_aqst_TradeAndOtherReceivablesNet_aaad839a-b8bc-4a60-98f6-36ea5ebbb3df" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_a0fe5cb2-e3e9-4b55-8430-4431ed276d2e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_7f853972-a48d-40f6-883e-7fff8fee8e2f" xlink:to="loc_us-gaap_InventoryNet_a0fe5cb2-e3e9-4b55-8430-4431ed276d2e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_d8d3b27f-bac5-42db-b74e-57cdbd2c3fba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_d8d3b27f-bac5-42db-b74e-57cdbd2c3fba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_0382829c-cded-4b1a-a084-fb00656f2b5f" xlink:href="aqst-20220630.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedNoncurrent_0382829c-cded-4b1a-a084-fb00656f2b5f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_e5b3d98f-70ba-4cba-b53d-bbbbe520b5c8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_e5b3d98f-70ba-4cba-b53d-bbbbe520b5c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_d4fce5d8-301a-4ec2-bc58-208a067db3c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_us-gaap_LiabilitiesCurrent_d4fce5d8-301a-4ec2-bc58-208a067db3c9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermLoansPayable_5cc059bb-7167-43f2-94aa-773faa869a68" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermLoansPayable"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_us-gaap_LongTermLoansPayable_5cc059bb-7167-43f2-94aa-773faa869a68" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_57e36937-3715-45ca-9f8e-ec373a63915c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_38ec38e5-f5e0-4f2d-9574-0c0db81c9717" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_57e36937-3715-45ca-9f8e-ec373a63915c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_7be86ab4-e985-4cf5-b13d-743a37e243f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_76edf4e7-a681-4b3f-81a4-3568fc58f466" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_7be86ab4-e985-4cf5-b13d-743a37e243f9" xlink:to="loc_us-gaap_CommonStockValue_76edf4e7-a681-4b3f-81a4-3568fc58f466" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_7233a018-585b-4a9c-9deb-49e20db04886" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_7be86ab4-e985-4cf5-b13d-743a37e243f9" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_7233a018-585b-4a9c-9deb-49e20db04886" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_3a913314-2668-4600-a0a0-e3ee7421cbe7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_7be86ab4-e985-4cf5-b13d-743a37e243f9" xlink:to="loc_us-gaap_AdditionalPaidInCapital_3a913314-2668-4600-a0a0-e3ee7421cbe7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_29875b4e-e46a-4b91-82e2-d64b0ed3db8d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_us-gaap_AccountsPayableCurrent_29875b4e-e46a-4b91-82e2-d64b0ed3db8d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LoansPayableCurrent_e97a27ea-9c09-4f5b-b5b5-4e0e3fc4b810" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LoansPayableCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_us-gaap_LoansPayableCurrent_e97a27ea-9c09-4f5b-b5b5-4e0e3fc4b810" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_cf75ed35-32da-4f64-9122-c00b48907859" xlink:href="aqst-20220630.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_cf75ed35-32da-4f64-9122-c00b48907859" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_4f92fa32-7095-4555-9541-e372e93e6d2a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_4f92fa32-7095-4555-9541-e372e93e6d2a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_cff9c704-99a6-4c09-8f30-0cad7a775e83" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_cff9c704-99a6-4c09-8f30-0cad7a775e83" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_625e4665-3119-49a8-b261-158fa0e1a504" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_67f76dd0-9df3-43a1-aee9-5ccb26013a0d" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_625e4665-3119-49a8-b261-158fa0e1a504" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="aqst-20220630.xsd#CondensedConsolidatedStatementsofOperationsandComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_6e0c25bd-27fb-4a26-8667-799161683550" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_83ec3c07-93f1-4b75-ae7d-a065bf221989" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_6e0c25bd-27fb-4a26-8667-799161683550" xlink:to="loc_us-gaap_NetIncomeLoss_83ec3c07-93f1-4b75-ae7d-a065bf221989" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f4a7aca9-5768-4577-802c-d35c0f4cfafb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_640db35f-2cf4-463a-b9f4-2c88d115da98" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestExpense"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f4a7aca9-5768-4577-802c-d35c0f4cfafb" xlink:to="loc_us-gaap_InterestExpense_640db35f-2cf4-463a-b9f4-2c88d115da98" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenues_a9ba948c-c886-4d35-9700-3d818cb8c270" xlink:href="aqst-20220630.xsd#aqst_InterestExpenseSaleOfFutureRevenues"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f4a7aca9-5768-4577-802c-d35c0f4cfafb" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenues_a9ba948c-c886-4d35-9700-3d818cb8c270" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestAndOtherIncomeNet_caf31333-d1ff-4419-ab50-a5e37b143480" xlink:href="aqst-20220630.xsd#aqst_InterestAndOtherIncomeNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f4a7aca9-5768-4577-802c-d35c0f4cfafb" xlink:to="loc_aqst_InterestAndOtherIncomeNet_caf31333-d1ff-4419-ab50-a5e37b143480" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_f81f3bda-6208-491a-b4a3-c3e17d8b4f1d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_f4a7aca9-5768-4577-802c-d35c0f4cfafb" xlink:to="loc_us-gaap_OperatingIncomeLoss_f81f3bda-6208-491a-b4a3-c3e17d8b4f1d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_da5474ec-91c6-4fa6-984d-f9e794592a3b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_1c55d460-a315-43da-8166-eba39a68beec" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpenses"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_da5474ec-91c6-4fa6-984d-f9e794592a3b" xlink:to="loc_us-gaap_CostsAndExpenses_1c55d460-a315-43da-8166-eba39a68beec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_2e9ba13e-feb3-4049-854f-c7935c330dd4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_da5474ec-91c6-4fa6-984d-f9e794592a3b" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_2e9ba13e-feb3-4049-854f-c7935c330dd4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostsAndExpenses_8038bc91-338c-4c46-9946-5093b0c007b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostsAndExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_7862bb95-ad8d-4962-94f9-78dcc101c187" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_8038bc91-338c-4c46-9946-5093b0c007b4" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_7862bb95-ad8d-4962-94f9-78dcc101c187" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_3424ce1f-89a0-48f1-b6e3-6fe11c8120f4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_8038bc91-338c-4c46-9946-5093b0c007b4" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_3424ce1f-89a0-48f1-b6e3-6fe11c8120f4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CostOfGoodsAndServicesSold_c4e8ca40-d6ae-4092-9519-c5639f5c0c40" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CostOfGoodsAndServicesSold"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CostsAndExpenses_8038bc91-338c-4c46-9946-5093b0c007b4" xlink:to="loc_us-gaap_CostOfGoodsAndServicesSold_c4e8ca40-d6ae-4092-9519-c5639f5c0c40" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_b143328a-8548-4a11-9811-ee0f413d7a12" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4e7a434f-3d72-4336-9d7d-f2356042a4d6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_b143328a-8548-4a11-9811-ee0f413d7a12" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_4e7a434f-3d72-4336-9d7d-f2356042a4d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_69383f62-be8a-4df8-af5f-83945cbb7a92" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_b143328a-8548-4a11-9811-ee0f413d7a12" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_69383f62-be8a-4df8-af5f-83945cbb7a92" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="aqst-20220630.xsd#CondensedConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/CondensedConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_d41fad5e-1adf-483e-9c51-8fa236c6f004" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fea35965-672b-43f2-999e-730af82009e1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_d41fad5e-1adf-483e-9c51-8fa236c6f004" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_fea35965-672b-43f2-999e-730af82009e1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_b4f0404a-a9bb-4021-b3cb-fe4e40b8e522" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_94faf6f0-3378-412f-bac5-21bc8f688c89" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_b4f0404a-a9bb-4021-b3cb-fe4e40b8e522" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_94faf6f0-3378-412f-bac5-21bc8f688c89" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_08cecddd-c0c0-49b3-898e-1c001603c96a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_b4f0404a-a9bb-4021-b3cb-fe4e40b8e522" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_08cecddd-c0c0-49b3-898e-1c001603c96a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8a037ac-4153-4619-9e26-e738c0b1a544" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_b4f0404a-a9bb-4021-b3cb-fe4e40b8e522" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8a037ac-4153-4619-9e26-e738c0b1a544" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_70a5e056-9599-4b5d-bf2e-99af8be909eb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_ShareBasedCompensation_70a5e056-9599-4b5d-bf2e-99af8be909eb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_781cf4e9-02e7-4f9f-9318-9cef9ba0de3a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_781cf4e9-02e7-4f9f-9318-9cef9ba0de3a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_bff9798c-8ecc-47e0-8a5d-437cf74d64f7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_DepreciationAndAmortization_bff9798c-8ecc-47e0-8a5d-437cf74d64f7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_286fd1ab-0b3d-4f0b-8625-6903a670525e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_286fd1ab-0b3d-4f0b-8625-6903a670525e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_97f0c98a-a3bf-4ac2-9a92-5717bec7d943" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_97f0c98a-a3bf-4ac2-9a92-5717bec7d943" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_854f9c08-60df-401b-acde-5c2d37c8810b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_854f9c08-60df-401b-acde-5c2d37c8810b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_5ab73067-12c2-4ba5-b961-2f3e76faa25a" xlink:href="aqst-20220630.xsd#aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_5ab73067-12c2-4ba5-b961-2f3e76faa25a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_8d5b97d7-166f-4e06-8417-15e740f63e14" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_NetIncomeLoss_8d5b97d7-166f-4e06-8417-15e740f63e14" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_0633b4e4-4a80-45e5-b582-21837f5df894" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_0633b4e4-4a80-45e5-b582-21837f5df894" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_474c9baf-6304-4858-be17-680fb8c18ad1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables"/>
    <link:calculationArc order="10" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_474c9baf-6304-4858-be17-680fb8c18ad1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_62642cb3-f304-445e-b0a5-ef97235b5cd0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_62642cb3-f304-445e-b0a5-ef97235b5cd0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_ef100750-f389-4b9f-adaa-985317366803" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="12" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_3aba7473-a70d-4475-b55b-6f3b096e8a1a" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_ef100750-f389-4b9f-adaa-985317366803" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuancePrivateOffering_9005026f-c009-479b-b93d-46a61dbcca43" xlink:href="aqst-20220630.xsd#aqst_ProceedsFromIssuancePrivateOffering"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_aqst_ProceedsFromIssuancePrivateOffering_9005026f-c009-479b-b93d-46a61dbcca43" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_0f110e2d-3ced-45da-8121-11f20b3d9ab5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_0f110e2d-3ced-45da-8121-11f20b3d9ab5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_e6b8e9f9-dda8-4c29-8609-38e4ba3ef298" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_e6b8e9f9-dda8-4c29-8609-38e4ba3ef298" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuanceAndExerciseOfWarrants_b482e282-edf9-43f1-8d53-cfdd93566c4b" xlink:href="aqst-20220630.xsd#aqst_ProceedsFromIssuanceAndExerciseOfWarrants"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_aqst_ProceedsFromIssuanceAndExerciseOfWarrants_b482e282-edf9-43f1-8d53-cfdd93566c4b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_3d804d30-0c23-41dc-9c66-5e35014dffe3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfDebtExtinguishmentCosts"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_3d804d30-0c23-41dc-9c66-5e35014dffe3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_7d1475d1-43bc-4d2c-8f49-610f5141f933" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceInitialPublicOffering"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_85d96598-6edc-405f-8270-3ae38d22ee9f" xlink:to="loc_us-gaap_ProceedsFromIssuanceInitialPublicOffering_7d1475d1-43bc-4d2c-8f49-610f5141f933" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RevenuesandTradeReceivablesNetTradeandOtherReceivablesNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_8c1800ee-7721-40b9-81e5-213dea4204e9" xlink:href="aqst-20220630.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_7b8bf8b5-49a5-4316-95b1-4f3bff36e615" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_8c1800ee-7721-40b9-81e5-213dea4204e9" xlink:to="loc_us-gaap_AccountsReceivableGross_7b8bf8b5-49a5-4316-95b1-4f3bff36e615" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherReceivablesGrossCurrent_9efb3b80-e393-4561-bc66-df0a1c75ead0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherReceivablesGrossCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_8c1800ee-7721-40b9-81e5-213dea4204e9" xlink:to="loc_us-gaap_OtherReceivablesGrossCurrent_9efb3b80-e393-4561-bc66-df0a1c75ead0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_9a6741a5-0ada-430a-8624-90df84870eba" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_8c1800ee-7721-40b9-81e5-213dea4204e9" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_9a6741a5-0ada-430a-8624-90df84870eba" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_f52a747b-c0ae-4515-8b42-9b9f5b214164" xlink:href="aqst-20220630.xsd#aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_8c1800ee-7721-40b9-81e5-213dea4204e9" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_f52a747b-c0ae-4515-8b42-9b9f5b214164" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/InventoriesNetDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#InventoriesNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/InventoriesNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_00ca3abd-806a-4d81-9062-7721f8e08254" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_bbd03abc-bcfa-4de2-a901-fda46f3dcca8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_00ca3abd-806a-4d81-9062-7721f8e08254" xlink:to="loc_us-gaap_InventoryRawMaterials_bbd03abc-bcfa-4de2-a901-fda46f3dcca8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InventoryPackingMaterialGross_c0b974c5-4cc3-4b21-905f-1d0eb20367cd" xlink:href="aqst-20220630.xsd#aqst_InventoryPackingMaterialGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_00ca3abd-806a-4d81-9062-7721f8e08254" xlink:to="loc_aqst_InventoryPackingMaterialGross_c0b974c5-4cc3-4b21-905f-1d0eb20367cd" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_e9107dcc-b673-4b6a-a4ce-b713a7ccf2b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_00ca3abd-806a-4d81-9062-7721f8e08254" xlink:to="loc_us-gaap_InventoryFinishedGoods_e9107dcc-b673-4b6a-a4ce-b713a7ccf2b4" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#PropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_de2b9da3-13da-4c7b-a6b8-fd68e0d56c12" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_e95e9939-cf38-4623-b0ec-0380a2136dd8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_de2b9da3-13da-4c7b-a6b8-fd68e0d56c12" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_e95e9939-cf38-4623-b0ec-0380a2136dd8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_ea7ad551-e93f-46fb-bd58-8515950db141" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_de2b9da3-13da-4c7b-a6b8-fd68e0d56c12" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_ea7ad551-e93f-46fb-bd58-8515950db141" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0681b51d-de08-44b5-8003-80c902e985d2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_66e031e0-b213-4b5e-b43c-60a931896d70" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0681b51d-de08-44b5-8003-80c902e985d2" xlink:to="loc_us-gaap_OperatingLeaseLiability_66e031e0-b213-4b5e-b43c-60a931896d70" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_3deebfb3-0147-4bad-9077-a78393b463cb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0681b51d-de08-44b5-8003-80c902e985d2" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_3deebfb3-0147-4bad-9077-a78393b463cb" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="simple" xlink:href="aqst-20220630.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails_1"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_f7293da7-4aa6-4f0b-8b46-0a7d3b3a4b52" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsRemainderOfFiscalYear_f7293da7-4aa6-4f0b-8b46-0a7d3b3a4b52" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_df030d8c-af64-48de-b892-49dc8d4b95ad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_df030d8c-af64-48de-b892-49dc8d4b95ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_69805662-a59d-48c1-8bec-5538774bba3e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_69805662-a59d-48c1-8bec-5538774bba3e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_40616826-768b-4f03-93a8-fba14c600be3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_40616826-768b-4f03-93a8-fba14c600be3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_af382760-e558-4dd8-a554-aae317846d80" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_d082b32d-e8f1-4abb-a08b-70067e94ec18" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_af382760-e558-4dd8-a554-aae317846d80" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#IntangibleAssetsNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IntangibleAssetsNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_99c3a156-fe5d-46e4-b24f-70c680ff3b25" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_37b34eb4-d4b0-49a4-9ae1-df694d83e09c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_99c3a156-fe5d-46e4-b24f-70c680ff3b25" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_37b34eb4-d4b0-49a4-9ae1-df694d83e09c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_20724aad-8b41-44ff-a8a3-1246fe5e199c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_99c3a156-fe5d-46e4-b24f-70c680ff3b25" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_20724aad-8b41-44ff-a8a3-1246fe5e199c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/OthernoncurrentAssetsDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#OthernoncurrentAssetsDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/OthernoncurrentAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_a9431e84-d0bb-415e-9d70-c80a41f5fc5c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_ae02ed89-04ba-4e58-afd5-06b5d2ef6246" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsMiscellaneousNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_a9431e84-d0bb-415e-9d70-c80a41f5fc5c" xlink:to="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_ae02ed89-04ba-4e58-afd5-06b5d2ef6246" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_CumulativeRoyaltyReceivable_ae1d70f9-54b3-4e70-b56a-ebcda0dbdae6" xlink:href="aqst-20220630.xsd#aqst_CumulativeRoyaltyReceivable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_a9431e84-d0bb-415e-9d70-c80a41f5fc5c" xlink:to="loc_aqst_CumulativeRoyaltyReceivable_ae1d70f9-54b3-4e70-b56a-ebcda0dbdae6" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#AccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_b25db556-004b-4e39-b845-6a28c3c2be25" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_55409f85-eaa0-4423-85e0-7529bf1baa6d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_b25db556-004b-4e39-b845-6a28c3c2be25" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_55409f85-eaa0-4423-85e0-7529bf1baa6d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_1e293d43-609b-4133-81b5-5968560787b6" xlink:href="aqst-20220630.xsd#aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_b25db556-004b-4e39-b845-6a28c3c2be25" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsAccountsPayableAndAccruedExpenses_1e293d43-609b-4133-81b5-5968560787b6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_159e3889-feb9-464a-b9ce-df363ec5ced6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_b25db556-004b-4e39-b845-6a28c3c2be25" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_159e3889-feb9-464a-b9ce-df363ec5ced6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_a3806605-6de0-4de7-97bc-1f5317f25f8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_b25db556-004b-4e39-b845-6a28c3c2be25" xlink:to="loc_us-gaap_TaxesPayableCurrent_a3806605-6de0-4de7-97bc-1f5317f25f8a" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20220630.xsd#A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_4009b335-1fb6-49a4-a657-53dcb7a3eff9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebt"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_5ed2aa17-da92-48d2-a45e-4a859e2e69a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_4009b335-1fb6-49a4-a657-53dcb7a3eff9" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_5ed2aa17-da92-48d2-a45e-4a859e2e69a7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_bc335a27-af56-4425-9367-b90b1a30207a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_4009b335-1fb6-49a4-a657-53dcb7a3eff9" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_bc335a27-af56-4425-9367-b90b1a30207a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_59034ef4-2814-41b7-942a-0d821136cfe5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_4009b335-1fb6-49a4-a657-53dcb7a3eff9" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_59034ef4-2814-41b7-942a-0d821136cfe5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_63d636e1-ebc4-4fb2-b451-c1249837c63c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_4009b335-1fb6-49a4-a657-53dcb7a3eff9" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalRemainderOfFiscalYear_63d636e1-ebc4-4fb2-b451-c1249837c63c" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
