<?xml version="1.0" encoding="UTF-8"?>

<!--XBRL Document Created with Wdesk from Workiva-->
<!--Copyright 2023 Workiva-->
<!--r:a50ccfd0-8a14-4c25-aa44-590c0d890ebb,g:b922e67f-7e88-4595-bec0-7eb47b678c0f-->
<link:linkbase xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd">
  <link:roleRef roleURI="http://aquestive.com/role/ConsolidatedBalanceSheets" xlink:type="simple" xlink:href="aqst-20221231.xsd#ConsolidatedBalanceSheets"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/ConsolidatedBalanceSheets" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_4bf5b85b-94bf-4ffc-9608-db0eef7a97a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityNoncurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityNoncurrent_4bf5b85b-94bf-4ffc-9608-db0eef7a97a0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermLoansPayable_a13630c0-8c86-4061-9779-1f99ef109fc9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermLoansPayable"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_us-gaap_LongTermLoansPayable_a13630c0-8c86-4061-9779-1f99ef109fc9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherLiabilitiesNoncurrent_8cf25aaf-0e52-4261-a396-f2028bcd7b47" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherLiabilitiesNoncurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_us-gaap_OtherLiabilitiesNoncurrent_8cf25aaf-0e52-4261-a396-f2028bcd7b47" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_43546108-7ce9-46c6-a5f2-d3688c8d8912" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_us-gaap_LiabilitiesCurrent_43546108-7ce9-46c6-a5f2-d3688c8d8912" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_LiabilitiesSaleOfFutureRevenueNet_1b0d8bc2-b16f-4296-8565-888246b87ace" xlink:href="aqst-20221231.xsd#aqst_LiabilitiesSaleOfFutureRevenueNet"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_aqst_LiabilitiesSaleOfFutureRevenueNet_1b0d8bc2-b16f-4296-8565-888246b87ace" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_67b9ed85-6b93-4850-aa11-de93b1b22281" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityNoncurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Liabilities_54c8156e-095e-4bd6-bf6d-5c7dca3a0b22" xlink:to="loc_us-gaap_OperatingLeaseLiabilityNoncurrent_67b9ed85-6b93-4850-aa11-de93b1b22281" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_a77af8ee-37a8-4743-9583-bffcc687794e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommonStockValue_80aadd77-5581-449a-82e5-b9d87a222c71" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommonStockValue"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a77af8ee-37a8-4743-9583-bffcc687794e" xlink:to="loc_us-gaap_CommonStockValue_80aadd77-5581-449a-82e5-b9d87a222c71" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AdditionalPaidInCapital_601545a9-1576-479a-b778-8ebd7c653e43" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AdditionalPaidInCapital"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a77af8ee-37a8-4743-9583-bffcc687794e" xlink:to="loc_us-gaap_AdditionalPaidInCapital_601545a9-1576-479a-b778-8ebd7c653e43" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RetainedEarningsAccumulatedDeficit_0c827ad5-5dc2-4cdc-aeff-5ba983174817" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RetainedEarningsAccumulatedDeficit"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_StockholdersEquity_a77af8ee-37a8-4743-9583-bffcc687794e" xlink:to="loc_us-gaap_RetainedEarningsAccumulatedDeficit_0c827ad5-5dc2-4cdc-aeff-5ba983174817" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ContractWithCustomerLiabilityCurrent_2f13bbdb-d6f4-4138-a39c-f5c52f04d4a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ContractWithCustomerLiabilityCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_us-gaap_ContractWithCustomerLiabilityCurrent_2f13bbdb-d6f4-4138-a39c-f5c52f04d4a7" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsPayableCurrent_259517b5-15a9-4ba2-97e1-4ba0b30bc288" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsPayableCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_us-gaap_AccountsPayableCurrent_259517b5-15a9-4ba2-97e1-4ba0b30bc288" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_db17c950-c82d-4893-984d-c190ad3821ad" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_us-gaap_AccruedLiabilitiesCurrent_db17c950-c82d-4893-984d-c190ad3821ad" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LinesOfCreditCurrent_e99fd510-9f24-4525-9160-e7184ed8cd54" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LinesOfCreditCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_us-gaap_LinesOfCreditCurrent_e99fd510-9f24-4525-9160-e7184ed8cd54" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiabilityCurrent_7dd01121-feb0-4d7c-ad24-b13f0fb77aef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiabilityCurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_us-gaap_OperatingLeaseLiabilityCurrent_7dd01121-feb0-4d7c-ad24-b13f0fb77aef" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_430e0db5-a710-4fb6-bcdf-e390f8f901d2" xlink:href="aqst-20221231.xsd#aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesCurrent_c4c57a4c-fdc2-4df3-aaae-68052dfd569a" xlink:to="loc_aqst_TransferOfFinancialAssetsAccountedForAsSalesAmountDerecognizedCurrent_430e0db5-a710-4fb6-bcdf-e390f8f901d2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Assets"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_0f8fe4d9-f1ee-4689-99c2-f92c405195b4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:to="loc_us-gaap_AssetsCurrent_0f8fe4d9-f1ee-4689-99c2-f92c405195b4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_ded1a982-d2d8-49f6-8b23-92a12653699a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentNet_ded1a982-d2d8-49f6-8b23-92a12653699a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1d4dfb55-c79b-41b5-b2d4-b6b0e5620985" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsNet_1d4dfb55-c79b-41b5-b2d4-b6b0e5620985" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseRightOfUseAsset_0472c066-63f4-4c7a-a62b-94b1ccd29085" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseRightOfUseAsset"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:to="loc_us-gaap_OperatingLeaseRightOfUseAsset_0472c066-63f4-4c7a-a62b-94b1ccd29085" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_4f9c0438-8e5a-4e23-ad3d-121c6aa12a8a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_Assets_c9e91339-9715-4b98-b7f8-5abd171cfcb2" xlink:to="loc_us-gaap_OtherAssetsNoncurrent_4f9c0438-8e5a-4e23-ad3d-121c6aa12a8a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AssetsCurrent_bfb31dd4-74a2-4cd6-91e5-94f3437ff362" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AssetsCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_89901430-9361-4695-8692-e5e57e654bf3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_bfb31dd4-74a2-4cd6-91e5-94f3437ff362" xlink:to="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalents_89901430-9361-4695-8692-e5e57e654bf3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_4c346ed8-0893-404e-b33c-90a036b60887" xlink:href="aqst-20221231.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_bfb31dd4-74a2-4cd6-91e5-94f3437ff362" xlink:to="loc_aqst_TradeAndOtherReceivablesNet_4c346ed8-0893-404e-b33c-90a036b60887" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_3aa0ea30-7046-44f7-8e8e-fe93f9a3fb1f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_bfb31dd4-74a2-4cd6-91e5-94f3437ff362" xlink:to="loc_us-gaap_InventoryNet_3aa0ea30-7046-44f7-8e8e-fe93f9a3fb1f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_6ab140fe-876b-4d67-982c-f95c14f8ab73" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PrepaidExpenseAndOtherAssetsCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AssetsCurrent_bfb31dd4-74a2-4cd6-91e5-94f3437ff362" xlink:to="loc_us-gaap_PrepaidExpenseAndOtherAssetsCurrent_6ab140fe-876b-4d67-982c-f95c14f8ab73" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LiabilitiesAndStockholdersEquity_43537905-b118-408a-adbf-b3567e9021be" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LiabilitiesAndStockholdersEquity"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_StockholdersEquity_766073dc-dcb9-4ff2-b86f-68e96f0ba3cf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_StockholdersEquity"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_43537905-b118-408a-adbf-b3567e9021be" xlink:to="loc_us-gaap_StockholdersEquity_766073dc-dcb9-4ff2-b86f-68e96f0ba3cf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_Liabilities_24b2f3fb-9763-4a11-a15a-2e2a77751cf2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_Liabilities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_43537905-b118-408a-adbf-b3567e9021be" xlink:to="loc_us-gaap_Liabilities_24b2f3fb-9763-4a11-a15a-2e2a77751cf2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CommitmentsAndContingencies_a519cf16-518d-4961-a258-7b7c617bce46" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CommitmentsAndContingencies"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LiabilitiesAndStockholdersEquity_43537905-b118-408a-adbf-b3567e9021be" xlink:to="loc_us-gaap_CommitmentsAndContingencies_a519cf16-518d-4961-a258-7b7c617bce46" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/ConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="simple" xlink:href="aqst-20221231.xsd#ConsolidatedStatementsofOperationsandComprehensiveLoss"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/ConsolidatedStatementsofOperationsandComprehensiveLoss" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_1ee44fcd-2b15-491e-b741-037abda9af5c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_1ee44fcd-2b15-491e-b741-037abda9af5c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_b2856894-85d8-4b85-95c8-35b2d2e770cf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:to="loc_us-gaap_OperatingIncomeLoss_b2856894-85d8-4b85-95c8-35b2d2e770cf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestExpense_9b5be055-91fa-46c9-ba6d-bc64c1d060b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestExpense"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:to="loc_us-gaap_InterestExpense_9b5be055-91fa-46c9-ba6d-bc64c1d060b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InterestAndOtherIncome_c5bec1b1-3ce1-45db-a425-204b91a1cbce" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InterestAndOtherIncome"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:to="loc_us-gaap_InterestAndOtherIncome_c5bec1b1-3ce1-45db-a425-204b91a1cbce" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenues_6c7a118e-b93b-4c30-ae17-cb31c6ac9508" xlink:href="aqst-20221231.xsd#aqst_InterestExpenseSaleOfFutureRevenues"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_5f3679fb-8588-435e-b4d9-ebe3b51f17a7" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenues_6c7a118e-b93b-4c30-ae17-cb31c6ac9508" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_a1093e98-ffd6-4488-983e-592870c4b7e2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_c1aab073-9403-4987-9b0c-adc927efdea4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_a1093e98-ffd6-4488-983e-592870c4b7e2" xlink:to="loc_us-gaap_IncomeLossFromContinuingOperationsBeforeIncomeTaxesExtraordinaryItemsNoncontrollingInterest_c1aab073-9403-4987-9b0c-adc927efdea4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncomeTaxExpenseBenefit_a424dc74-0d46-4eb3-bc12-b73faade5cdf" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncomeTaxExpenseBenefit"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetIncomeLoss_a1093e98-ffd6-4488-983e-592870c4b7e2" xlink:to="loc_us-gaap_IncomeTaxExpenseBenefit_a424dc74-0d46-4eb3-bc12-b73faade5cdf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_9385f5ed-16aa-4189-b0fe-1703e11b2751" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingExpenses"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ManufactureAndSupplyCosts_6b2e0fba-e3d6-481e-a00b-39402a1c1c3d" xlink:href="aqst-20221231.xsd#aqst_ManufactureAndSupplyCosts"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_9385f5ed-16aa-4189-b0fe-1703e11b2751" xlink:to="loc_aqst_ManufactureAndSupplyCosts_6b2e0fba-e3d6-481e-a00b-39402a1c1c3d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ResearchAndDevelopmentExpense_7dd0217a-d32b-4b48-b7a2-52f95bdc705a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ResearchAndDevelopmentExpense"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_9385f5ed-16aa-4189-b0fe-1703e11b2751" xlink:to="loc_us-gaap_ResearchAndDevelopmentExpense_7dd0217a-d32b-4b48-b7a2-52f95bdc705a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_SellingGeneralAndAdministrativeExpense_e5dee710-deae-48cd-b8b5-2ed043c2e21e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_SellingGeneralAndAdministrativeExpense"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingExpenses_9385f5ed-16aa-4189-b0fe-1703e11b2751" xlink:to="loc_us-gaap_SellingGeneralAndAdministrativeExpense_e5dee710-deae-48cd-b8b5-2ed043c2e21e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingIncomeLoss_5fcc0554-1647-466e-ba4d-410137063a06" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingIncomeLoss"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c534ba31-2916-453f-9c3c-68ebe16c3bb8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_5fcc0554-1647-466e-ba4d-410137063a06" xlink:to="loc_us-gaap_RevenueFromContractWithCustomerExcludingAssessedTax_c534ba31-2916-453f-9c3c-68ebe16c3bb8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingExpenses_ab6ebc7b-b8fe-4ed5-bdc5-9538bc4b3b0f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingExpenses"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OperatingIncomeLoss_5fcc0554-1647-466e-ba4d-410137063a06" xlink:to="loc_us-gaap_OperatingExpenses_ab6ebc7b-b8fe-4ed5-bdc5-9538bc4b3b0f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ComprehensiveIncomeNetOfTax_b81844e0-6147-4051-abc1-d8b619ed156d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ComprehensiveIncomeNetOfTax"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_f69431b5-fa7a-473a-8b3e-81fce123949c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_ComprehensiveIncomeNetOfTax_b81844e0-6147-4051-abc1-d8b619ed156d" xlink:to="loc_us-gaap_NetIncomeLoss_f69431b5-fa7a-473a-8b3e-81fce123949c" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/ConsolidatedStatementsofCashFlows" xlink:type="simple" xlink:href="aqst-20221231.xsd#ConsolidatedStatementsofCashFlows"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/ConsolidatedStatementsofCashFlows" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_25ebda13-3979-4602-993e-8936185dd612" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsOfDebtExtinguishmentCosts"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_us-gaap_PaymentsOfDebtExtinguishmentCosts_25ebda13-3979-4602-993e-8936185dd612" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_fa5d2d17-6d47-47f2-adfb-5d0889b4590f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_us-gaap_ProceedsFromIssuanceOfSharesUnderIncentiveAndShareBasedCompensationPlans_fa5d2d17-6d47-47f2-adfb-5d0889b4590f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromStockOptionsExercised_981128be-7fe4-42d4-a8b3-2470704a80a3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromStockOptionsExercised"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_us-gaap_ProceedsFromStockOptionsExercised_981128be-7fe4-42d4-a8b3-2470704a80a3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuanceOfPublicOfferingIssuanceOfWarrantsAndWarrantExercises_aa0cad3f-0026-437b-b3a6-9c7776b55b4b" xlink:href="aqst-20221231.xsd#aqst_ProceedsFromIssuanceOfPublicOfferingIssuanceOfWarrantsAndWarrantExercises"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_aqst_ProceedsFromIssuanceOfPublicOfferingIssuanceOfWarrantsAndWarrantExercises_aa0cad3f-0026-437b-b3a6-9c7776b55b4b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_14245e6a-8ffd-4162-9d72-89fcee334915" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_us-gaap_PaymentsRelatedToTaxWithholdingForShareBasedCompensation_14245e6a-8ffd-4162-9d72-89fcee334915" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_ProceedsFromIssuancePrivateOffering_aacf9551-2986-4dfe-9ebf-eb783b34e8f2" xlink:href="aqst-20221231.xsd#aqst_ProceedsFromIssuancePrivateOffering"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_b8169d25-c884-4f08-ae47-2b69242632c8" xlink:to="loc_aqst_ProceedsFromIssuancePrivateOffering_aacf9551-2986-4dfe-9ebf-eb783b34e8f2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_77bf70fd-b7e0-4ee6-8b40-da01322afb6b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5a6f4acf-1330-42e8-884f-3eab61741114" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_77bf70fd-b7e0-4ee6-8b40-da01322afb6b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_5a6f4acf-1330-42e8-884f-3eab61741114" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_84a4ce9e-3b59-4d13-a571-cd2037f601a0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_77bf70fd-b7e0-4ee6-8b40-da01322afb6b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_84a4ce9e-3b59-4d13-a571-cd2037f601a0" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_a2307400-7133-4c68-be7f-8650e7188c1b" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInFinancingActivities"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_CashCashEquivalentsRestrictedCashAndRestrictedCashEquivalentsPeriodIncreaseDecreaseIncludingExchangeRateEffect_77bf70fd-b7e0-4ee6-8b40-da01322afb6b" xlink:to="loc_us-gaap_NetCashProvidedByUsedInFinancingActivities_a2307400-7133-4c68-be7f-8650e7188c1b" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInOperatingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInInventories_143acc32-6926-421a-81eb-80eb91da6247" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInInventories"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInInventories_143acc32-6926-421a-81eb-80eb91da6247" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ShareBasedCompensation_d0b19e19-d4bb-4146-84d5-44d32c50e9b5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ShareBasedCompensation"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_ShareBasedCompensation_d0b19e19-d4bb-4146-84d5-44d32c50e9b5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsPayable_402a9f0e-8683-453b-bc73-8b6ba2f81192" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsPayable"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsPayable_402a9f0e-8683-453b-bc73-8b6ba2f81192" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_102563cc-2db1-486d-b5c1-6b0f1447504a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_GainsLossesOnExtinguishmentOfDebt"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_GainsLossesOnExtinguishmentOfDebt_102563cc-2db1-486d-b5c1-6b0f1447504a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_0afa45ca-aceb-4a33-b7b0-fa83f96238da" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets"/>
    <link:calculationArc order="5" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInPrepaidDeferredExpenseAndOtherAssets_0afa45ca-aceb-4a33-b7b0-fa83f96238da" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_39e059da-018d-4028-aa60-20766a2e56f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AmortizationOfFinancingCostsAndDiscounts"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_AmortizationOfFinancingCostsAndDiscounts_39e059da-018d-4028-aa60-20766a2e56f9" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_74403c0d-d059-4b56-8415-6741df6129b8" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInAccruedLiabilitiesAndOtherOperatingLiabilities_74403c0d-d059-4b56-8415-6741df6129b8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_9101f350-15e8-4ab4-bf89-348d672bd7c5" xlink:href="aqst-20221231.xsd#aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_aqst_InterestExpenseSaleOfFutureRevenuesExcludingAmortization_9101f350-15e8-4ab4-bf89-348d672bd7c5" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_43f7dabf-d533-4d7a-9652-00197f7f886c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInDeferredRevenue"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInDeferredRevenue_43f7dabf-d533-4d7a-9652-00197f7f886c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DepreciationAndAmortization_6999a18a-716d-4781-b0e4-66932a7918a2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DepreciationAndAmortization"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_DepreciationAndAmortization_6999a18a-716d-4781-b0e4-66932a7918a2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherNoncashIncomeExpense_5471029d-08e7-4c0a-b93b-48671784e947" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherNoncashIncomeExpense"/>
    <link:calculationArc order="11" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_OtherNoncashIncomeExpense_5471029d-08e7-4c0a-b93b-48671784e947" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_ProceedsFromRepaymentsOfNotesPayable_38689b0b-6403-4c93-941a-2bd4986ebaeb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_ProceedsFromRepaymentsOfNotesPayable"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_ProceedsFromRepaymentsOfNotesPayable_38689b0b-6403-4c93-941a-2bd4986ebaeb" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_ff1002b4-7805-4401-8c89-3189cd40df39" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables"/>
    <link:calculationArc order="13" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_IncreaseDecreaseInAccountsAndOtherReceivables_ff1002b4-7805-4401-8c89-3189cd40df39" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetIncomeLoss_8a24e746-a8f3-4a78-b046-8f921a26cd0e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetIncomeLoss"/>
    <link:calculationArc order="14" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInOperatingActivities_8002d18f-5cdd-404a-9d5e-980572e66d40" xlink:to="loc_us-gaap_NetIncomeLoss_8a24e746-a8f3-4a78-b046-8f921a26cd0e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_da3349a5-2573-4bcb-9a7a-dd29d0f678fb" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_NetCashProvidedByUsedInInvestingActivities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_93764f7a-0c50-44e1-8d58-621762ad2fb2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquirePropertyPlantAndEquipment"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_da3349a5-2573-4bcb-9a7a-dd29d0f678fb" xlink:to="loc_us-gaap_PaymentsToAcquirePropertyPlantAndEquipment_93764f7a-0c50-44e1-8d58-621762ad2fb2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PaymentsToAcquireIntangibleAssets_a40b6b66-78ed-4566-a356-42ac9a5078f9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PaymentsToAcquireIntangibleAssets"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_NetCashProvidedByUsedInInvestingActivities_da3349a5-2573-4bcb-9a7a-dd29d0f678fb" xlink:to="loc_us-gaap_PaymentsToAcquireIntangibleAssets_a40b6b66-78ed-4566-a356-42ac9a5078f9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RevenuesandTradeReceivablesNetAccountsReceivableNetDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#RevenuesandTradeReceivablesNetAccountsReceivableNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RevenuesandTradeReceivablesNetAccountsReceivableNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TradeAndOtherReceivablesNet_f3a793f6-effb-4ac3-b07c-aefc8d388cca" xlink:href="aqst-20221231.xsd#aqst_TradeAndOtherReceivablesNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccountsReceivableGross_879074c9-9748-4adf-a866-90e1c9c73216" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccountsReceivableGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_f3a793f6-effb-4ac3-b07c-aefc8d388cca" xlink:to="loc_us-gaap_AccountsReceivableGross_879074c9-9748-4adf-a866-90e1c9c73216" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_f4ca1301-6393-4774-827d-fae5fba83506" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AllowanceForDoubtfulAccountsReceivable"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_f3a793f6-effb-4ac3-b07c-aefc8d388cca" xlink:to="loc_us-gaap_AllowanceForDoubtfulAccountsReceivable_f4ca1301-6393-4774-827d-fae5fba83506" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherReceivablesGrossCurrent_104b2595-7b01-4256-b8c4-6774167f5a42" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherReceivablesGrossCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_f3a793f6-effb-4ac3-b07c-aefc8d388cca" xlink:to="loc_us-gaap_OtherReceivablesGrossCurrent_104b2595-7b01-4256-b8c4-6774167f5a42" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_22e85b14-f0cd-4558-8729-5398ec7e63bf" xlink:href="aqst-20221231.xsd#aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet"/>
    <link:calculationArc order="4" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_aqst_TradeAndOtherReceivablesNet_f3a793f6-effb-4ac3-b07c-aefc8d388cca" xlink:to="loc_aqst_AccountsReceivableAllowancesAndAccrualsTradeAndOtherReceivablesNet_22e85b14-f0cd-4558-8729-5398ec7e63bf" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/InventoryDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#InventoryDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/InventoryDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryNet_07709234-db76-49c3-a609-66545b74c353" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryRawMaterials_43909cab-5cb3-4a49-9177-1dab1e88185d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryRawMaterials"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_07709234-db76-49c3-a609-66545b74c353" xlink:to="loc_us-gaap_InventoryRawMaterials_43909cab-5cb3-4a49-9177-1dab1e88185d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_InventoryPackingMaterialGross_8eb3a94d-d746-4eb9-93b9-2aee3a2681ec" xlink:href="aqst-20221231.xsd#aqst_InventoryPackingMaterialGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_07709234-db76-49c3-a609-66545b74c353" xlink:to="loc_aqst_InventoryPackingMaterialGross_8eb3a94d-d746-4eb9-93b9-2aee3a2681ec" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_InventoryFinishedGoods_280620cd-ed0f-4af7-8ea6-c67a176aa2f0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_InventoryFinishedGoods"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_InventoryNet_07709234-db76-49c3-a609-66545b74c353" xlink:to="loc_us-gaap_InventoryFinishedGoods_280620cd-ed0f-4af7-8ea6-c67a176aa2f0" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#PropertyandEquipmentNetDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/PropertyandEquipmentNetDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentNet_ec5330fd-d6e8-41f2-92e2-a47e3cdc6b77" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_PropertyPlantAndEquipmentGross_a5a790a8-5a12-457f-aa5e-57b4222489d1" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_PropertyPlantAndEquipmentGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_ec5330fd-d6e8-41f2-92e2-a47e3cdc6b77" xlink:to="loc_us-gaap_PropertyPlantAndEquipmentGross_a5a790a8-5a12-457f-aa5e-57b4222489d1" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_c050369c-9387-42c5-a6db-81bf5428b347" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_PropertyPlantAndEquipmentNet_ec5330fd-d6e8-41f2-92e2-a47e3cdc6b77" xlink:to="loc_us-gaap_AccumulatedDepreciationDepletionAndAmortizationPropertyPlantAndEquipment_c050369c-9387-42c5-a6db-81bf5428b347" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_c14e74fc-bcaa-4847-9287-68004168f44d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFour_c14e74fc-bcaa-4847-9287-68004168f44d" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_7ceeeaf6-88f0-4ff7-9da9-61cdba9cd049" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueAfterYearFive_7ceeeaf6-88f0-4ff7-9da9-61cdba9cd049" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_cc6e7b1e-0efa-4997-9ca3-fde0fa006e7f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueNextTwelveMonths_cc6e7b1e-0efa-4997-9ca3-fde0fa006e7f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_c43ad1ec-83f6-4cb7-ae0a-bd64af75832e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearTwo_c43ad1ec-83f6-4cb7-ae0a-bd64af75832e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_8d27998f-2df8-40f8-90e7-36cb8b475ead" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearThree_8d27998f-2df8-40f8-90e7-36cb8b475ead" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_68425fbf-cc58-4411-9df7-9aa7d0fcc8b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_0a2f14f0-9fc2-4742-a183-d783123413e5" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDueYearFive_68425fbf-cc58-4411-9df7-9aa7d0fcc8b3" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="simple" xlink:href="aqst-20221231.xsd#RightofUseAssetsandLeaseObligationsMaturitiesDetails_1"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/RightofUseAssetsandLeaseObligationsMaturitiesDetails_1" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e72080fb-20f1-49cb-8fc4-8b61602b563f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_e55dd95c-148e-4ea4-82c2-cf3040572141" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e72080fb-20f1-49cb-8fc4-8b61602b563f" xlink:to="loc_us-gaap_LesseeOperatingLeaseLiabilityUndiscountedExcessAmount_e55dd95c-148e-4ea4-82c2-cf3040572141" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OperatingLeaseLiability_cd1d6e30-43a1-4e5c-8a2c-34709c79ac0d" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OperatingLeaseLiability"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LesseeOperatingLeaseLiabilityPaymentsDue_e72080fb-20f1-49cb-8fc4-8b61602b563f" xlink:to="loc_us-gaap_OperatingLeaseLiability_cd1d6e30-43a1-4e5c-8a2c-34709c79ac0d" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IntangibleAssetsNetandOthernoncurrentassetsScheduleofFiniteLivedIntangibleAssetsDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#IntangibleAssetsNetandOthernoncurrentassetsScheduleofFiniteLivedIntangibleAssetsDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IntangibleAssetsNetandOthernoncurrentassetsScheduleofFiniteLivedIntangibleAssetsDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsNet_ec8ddb2b-91e7-4443-96c7-50ad42b97984" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsGross_e2847ca2-42b2-470f-962c-989593b60a7f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsGross"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_ec8ddb2b-91e7-4443-96c7-50ad42b97984" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsGross_e2847ca2-42b2-470f-962c-989593b60a7f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_35743a65-431a-424f-9206-08effa6dcfff" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization"/>
    <link:calculationArc order="2" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_FiniteLivedIntangibleAssetsNet_ec8ddb2b-91e7-4443-96c7-50ad42b97984" xlink:to="loc_us-gaap_FiniteLivedIntangibleAssetsAccumulatedAmortization_35743a65-431a-424f-9206-08effa6dcfff" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsNoncurrent_77f6d23f-bc1a-482f-aabe-50f759773f64" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsNoncurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_RoyaltyReceivable_fccde7f9-2e32-40ec-81af-c437fc8d3daa" xlink:href="aqst-20221231.xsd#aqst_RoyaltyReceivable"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_77f6d23f-bc1a-482f-aabe-50f759773f64" xlink:to="loc_aqst_RoyaltyReceivable_fccde7f9-2e32-40ec-81af-c437fc8d3daa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_26790a45-223d-4196-9f9d-ad9132e818c9" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAssetsMiscellaneousNoncurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_OtherAssetsNoncurrent_77f6d23f-bc1a-482f-aabe-50f759773f64" xlink:to="loc_us-gaap_OtherAssetsMiscellaneousNoncurrent_26790a45-223d-4196-9f9d-ad9132e818c9" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#AccruedExpensesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/AccruedExpensesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedLiabilitiesCurrent_3d38d40f-855f-4c9f-991d-22792feef221" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedLiabilitiesCurrent"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_OtherAccruedLiabilitiesCurrent_747e58f7-dcc1-4134-9c83-66bcc3544ca4" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_OtherAccruedLiabilitiesCurrent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_3d38d40f-855f-4c9f-991d-22792feef221" xlink:to="loc_us-gaap_OtherAccruedLiabilitiesCurrent_747e58f7-dcc1-4134-9c83-66bcc3544ca4" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_aea62229-d700-4564-9a6c-6022ad419066" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EmployeeRelatedLiabilitiesCurrent"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_3d38d40f-855f-4c9f-991d-22792feef221" xlink:to="loc_us-gaap_EmployeeRelatedLiabilitiesCurrent_aea62229-d700-4564-9a6c-6022ad419066" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_TaxesPayableCurrent_f4ad7f68-0d2e-4e7b-9fec-b7166f94f2d6" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_TaxesPayableCurrent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_3d38d40f-855f-4c9f-991d-22792feef221" xlink:to="loc_us-gaap_TaxesPayableCurrent_f4ad7f68-0d2e-4e7b-9fec-b7166f94f2d6" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_AccruedMarketingCostsCurrent_ab4f8dd9-934b-46c5-92f2-a598c50ce7d0" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_AccruedMarketingCostsCurrent"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_AccruedLiabilitiesCurrent_3d38d40f-855f-4c9f-991d-22792feef221" xlink:to="loc_us-gaap_AccruedMarketingCostsCurrent_ab4f8dd9-934b-46c5-92f2-a598c50ce7d0" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/A125SeniorSecuredNotesandLoansPayableDebtMaturitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebt_25208429-fc0b-44a2-9d3e-49b96ff6e505" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebt"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_1b479d4e-b518-49e9-a93e-ccd2ffb9a73e" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_25208429-fc0b-44a2-9d3e-49b96ff6e505" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearThree_1b479d4e-b518-49e9-a93e-ccd2ffb9a73e" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_4c87320c-3aa5-4e55-947a-3c74020264e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_25208429-fc0b-44a2-9d3e-49b96ff6e505" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInYearTwo_4c87320c-3aa5-4e55-947a-3c74020264e3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_92f4e509-958c-4c4e-91a7-da76796a8516" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_LongTermDebt_25208429-fc0b-44a2-9d3e-49b96ff6e505" xlink:to="loc_us-gaap_LongTermDebtMaturitiesRepaymentsOfPrincipalInNextTwelveMonths_92f4e509-958c-4c4e-91a7-da76796a8516" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IncomeTaxesDeferredTaxAssetsandLiabilitiesDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#IncomeTaxesDeferredTaxAssetsandLiabilitiesDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IncomeTaxesDeferredTaxAssetsandLiabilitiesDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsGross"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities_b28d4e2c-b7c5-491b-bd5f-b85e510efc94" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccrualsAccruedLiabilities_b28d4e2c-b7c5-491b-bd5f-b85e510efc94" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost_9ea6d75d-aa59-4715-83b7-9cd6516c444c" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseCompensationAndBenefitsShareBasedCompensationCost_9ea6d75d-aa59-4715-83b7-9cd6516c444c" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwards_bfd88b50-3744-419e-936b-a3fbab21a31a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsOperatingLossCarryforwards"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsOperatingLossCarryforwards_bfd88b50-3744-419e-936b-a3fbab21a31a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_DeferredTaxAssetsSaleOfFutureRevenue_3a9351f7-3f4e-4635-9a3b-a661b5ddda22" xlink:href="aqst-20221231.xsd#aqst_DeferredTaxAssetsSaleOfFutureRevenue"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_aqst_DeferredTaxAssetsSaleOfFutureRevenue_3a9351f7-3f4e-4635-9a3b-a661b5ddda22" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsGoodwillAndIntangibleAssets_0b1b666f-4f08-4cc5-845d-f38d126189ef" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsGoodwillAndIntangibleAssets"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsGoodwillAndIntangibleAssets_0b1b666f-4f08-4cc5-845d-f38d126189ef" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwards_7443fc8d-7ae6-41f7-b388-8a62df96d172" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsTaxCreditCarryforwards"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwards_7443fc8d-7ae6-41f7-b388-8a62df96d172" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_TaxCutsAndJobsActOf2017DeferredTaxAssetsInterestLimitation_52f1e4d5-63b5-49c3-87b8-bdf08c8378bf" xlink:href="aqst-20221231.xsd#aqst_TaxCutsAndJobsActOf2017DeferredTaxAssetsInterestLimitation"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_aqst_TaxCutsAndJobsActOf2017DeferredTaxAssetsInterestLimitation_52f1e4d5-63b5-49c3-87b8-bdf08c8378bf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals_0a5967e0-9c14-48fb-8754-131d69dff4b3" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxDeferredExpenseReservesAndAccruals_0a5967e0-9c14-48fb-8754-131d69dff4b3" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsPropertyPlantAndEquipment_e08d6a2d-f18d-48f6-843f-37dd47b79ba2" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsPropertyPlantAndEquipment"/>
    <link:calculationArc order="9" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsPropertyPlantAndEquipment_e08d6a2d-f18d-48f6-843f-37dd47b79ba2" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_66d47714-1458-41bc-983e-6223e53f1304" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch"/>
    <link:calculationArc order="10" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsTaxCreditCarryforwardsResearch_66d47714-1458-41bc-983e-6223e53f1304" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_DeferredTaxAssetsAccountsReceivable_5effe28a-c359-4f3a-b209-39d8a776cd76" xlink:href="aqst-20221231.xsd#aqst_DeferredTaxAssetsAccountsReceivable"/>
    <link:calculationArc order="11" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_aqst_DeferredTaxAssetsAccountsReceivable_5effe28a-c359-4f3a-b209-39d8a776cd76" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsOther_df310400-c100-4168-b0fb-e567b0883b95" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsOther"/>
    <link:calculationArc order="12" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsOther_df310400-c100-4168-b0fb-e567b0883b95" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsInventory_97cf77ca-2d24-4c97-b13d-a84cba0ba2df" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsInventory"/>
    <link:calculationArc order="13" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsGross_6f733dc4-73ea-452e-bb30-c641508f66e3" xlink:to="loc_us-gaap_DeferredTaxAssetsInventory_97cf77ca-2d24-4c97-b13d-a84cba0ba2df" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilities_2813bd54-10ad-435a-aa38-c5257650a461" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredIncomeTaxLiabilities"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxLiabilitiesPrepaidExpenses_c5e26ade-7a95-438a-956b-da69ae7eff35" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxLiabilitiesPrepaidExpenses"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredIncomeTaxLiabilities_2813bd54-10ad-435a-aa38-c5257650a461" xlink:to="loc_us-gaap_DeferredTaxLiabilitiesPrepaidExpenses_c5e26ade-7a95-438a-956b-da69ae7eff35" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_DeferredTaxLiabilities481aAdjustment_cb1ac21c-603b-47c8-baaa-dc83558941bf" xlink:href="aqst-20221231.xsd#aqst_DeferredTaxLiabilities481aAdjustment"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredIncomeTaxLiabilities_2813bd54-10ad-435a-aa38-c5257650a461" xlink:to="loc_aqst_DeferredTaxLiabilities481aAdjustment_cb1ac21c-603b-47c8-baaa-dc83558941bf" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsNet_e8877da4-6a69-43ce-aeeb-1cc29bbbe0cd" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsNet"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredIncomeTaxLiabilities_6372e2c7-1d9d-409b-84d7-1f5cfce88389" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredIncomeTaxLiabilities"/>
    <link:calculationArc order="1" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsNet_e8877da4-6a69-43ce-aeeb-1cc29bbbe0cd" xlink:to="loc_us-gaap_DeferredIncomeTaxLiabilities_6372e2c7-1d9d-409b-84d7-1f5cfce88389" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsGross_d752f9d8-3b74-4031-8e7c-ecbc59ad08fa" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsGross"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsNet_e8877da4-6a69-43ce-aeeb-1cc29bbbe0cd" xlink:to="loc_us-gaap_DeferredTaxAssetsGross_d752f9d8-3b74-4031-8e7c-ecbc59ad08fa" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_DeferredTaxAssetsValuationAllowance_a977ecc4-b128-4230-b377-95e3e3e22eb5" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_DeferredTaxAssetsValuationAllowance"/>
    <link:calculationArc order="3" weight="-1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_DeferredTaxAssetsNet_e8877da4-6a69-43ce-aeeb-1cc29bbbe0cd" xlink:to="loc_us-gaap_DeferredTaxAssetsValuationAllowance_a977ecc4-b128-4230-b377-95e3e3e22eb5" xlink:type="arc"/>
  </link:calculationLink>
  <link:roleRef roleURI="http://aquestive.com/role/IncomeTaxesStatutoryFederalIncomeTaxRateReconciliationDetails" xlink:type="simple" xlink:href="aqst-20221231.xsd#IncomeTaxesStatutoryFederalIncomeTaxRateReconciliationDetails"/>
  <link:calculationLink xlink:role="http://aquestive.com/role/IncomeTaxesStatutoryFederalIncomeTaxRateReconciliationDetails" xlink:type="extended">
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateContinuingOperations"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_EffectiveIncomeTaxRateReconciliationTaxCreditReturnToProvisionPercent_bb1b851c-7dc7-407b-a032-2a4aa04f8d55" xlink:href="aqst-20221231.xsd#aqst_EffectiveIncomeTaxRateReconciliationTaxCreditReturnToProvisionPercent"/>
    <link:calculationArc order="1" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_aqst_EffectiveIncomeTaxRateReconciliationTaxCreditReturnToProvisionPercent_bb1b851c-7dc7-407b-a032-2a4aa04f8d55" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes_aa04182f-e256-4f61-91bf-d55676ed4abc" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes"/>
    <link:calculationArc order="2" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationStateAndLocalIncomeTaxes_aa04182f-e256-4f61-91bf-d55676ed4abc" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_aqst_EffectiveIncomeTaxRateReconciliationPermanentDifferencesPercent_5b17cfb7-9f8f-4d60-ad2f-db96a87383c8" xlink:href="aqst-20221231.xsd#aqst_EffectiveIncomeTaxRateReconciliationPermanentDifferencesPercent"/>
    <link:calculationArc order="3" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_aqst_EffectiveIncomeTaxRateReconciliationPermanentDifferencesPercent_5b17cfb7-9f8f-4d60-ad2f-db96a87383c8" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate_370e160e-1d50-4fee-a66f-4f5cd219b79a" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate"/>
    <link:calculationArc order="4" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInEnactedTaxRate_370e160e-1d50-4fee-a66f-4f5cd219b79a" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments_abc1e73a-ab89-4693-ba0f-1d593ac7c021" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments"/>
    <link:calculationArc order="5" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationOtherAdjustments_abc1e73a-ab89-4693-ba0f-1d593ac7c021" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_b8cbe8f5-20d1-47d1-8f13-45a585edf211" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate"/>
    <link:calculationArc order="6" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationAtFederalStatutoryIncomeTaxRate_b8cbe8f5-20d1-47d1-8f13-45a585edf211" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_dfb7dd60-1ba5-4c25-be3f-fe2d8d749c9f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance"/>
    <link:calculationArc order="7" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationChangeInDeferredTaxAssetsValuationAllowance_dfb7dd60-1ba5-4c25-be3f-fe2d8d749c9f" xlink:type="arc"/>
    <link:loc xlink:type="locator" xlink:label="loc_us-gaap_EffectiveIncomeTaxRateReconciliationTaxCreditsResearch_f8246aa3-f8da-48e0-a0b9-d306bf214e7f" xlink:href="https://xbrl.fasb.org/us-gaap/2022/elts/us-gaap-2022.xsd#us-gaap_EffectiveIncomeTaxRateReconciliationTaxCreditsResearch"/>
    <link:calculationArc order="8" weight="1.0" xlink:arcrole="http://www.xbrl.org/2003/arcrole/summation-item" xlink:from="loc_us-gaap_EffectiveIncomeTaxRateContinuingOperations_d01c9a59-3bb3-4249-b0ac-6df35e0e4b79" xlink:to="loc_us-gaap_EffectiveIncomeTaxRateReconciliationTaxCreditsResearch_f8246aa3-f8da-48e0-a0b9-d306bf214e7f" xlink:type="arc"/>
  </link:calculationLink>
</link:linkbase>
